Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 31;43(4):303–309. doi: 10.5115/acb.2010.43.4.303

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010. Anatomy and Cell Biology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 2 — Knock-down of NFAT2 by infection of adenovirus expressing microRNA specific for NFAT2. (A) The B16 cells were transduced with adenovirus expressing microRNA specific for NFAT2 (Ad/miNFAT2) at the 10 multiplicity of infection (MOI) for 6 h. After washing twice with PBS, cells were refed with growth medium and incubated for 24 h. The expression of NFAT2 was verified by Western blot using the anti-NFAT2 antibody and anti-actin antibody. Adenovirus expressing scrambled microRNA (Ad/miScr) was used as a negative control. (B) Cell survival was measured by WTS assay. Data are expressed as percentage of control (Ad/miScr at 1 day). The mean values±SEM are averages of triplicate measurements. ^*Statistically significant difference (P<0.05).