Fig. 2.

Induction of apoptosis in HeLa cells by suberoylanilide hydroxamic acid (SAHA), sirtinol or cisplatin, alone and in combination. Cells were incubated for 48 hr with 2.5 µm SAHA (B), 25 µm sirtinol (F) or 10 µm cisplatin (C and G) alone, or with 2.5 µm SAHA/10 µm cisplatin (D) or 25 µm sirtinol/10 µm cisplatin (H) combinations. (A) and (E) are controls. The cells were sampled, fixed and stained with DAPI; stained nuclei were then observed under a fluorescence microscope using a blue filter.