Figure 1. Circulating and placental VEGF levels are decreased in BPH/5.

A) Summary of circulating free VEGF as determined by ELISA in non-pregnant (NP) and at early and mid-gestation C57 and BPH/5 (n=4-6 per group). B) Summary of real-time qPCR analysis of placental Vegf mRNA levels at different days of gestation (n=5 per group). Data are expressed relative to C57 at e9.5 C) Representative Western blot of VEGF protein levels in placenta samples from C57 (C) and BPH/5 (B) at early and mid-gestation. β-actin served as loading control. D) Summary of Western blot quantification (n=3 per group at each time-point) normalized to actin and expressed relative to C57 early. *p<0.05 vs NP in matched strain; †p<0.05 vs. time-matched C57.