Table 2.
Experimentally determined net (→) and exchange (↔) fluxes and 95% flux confidence intervals for the A549 carcinoma cell
Glycolysis | ||
---|---|---|
Reaction | Flux | Interval |
Glucext → G6P | 38.6 | [34.7, 43.2] |
G6P → F6P | 32.4 | [29.7, 35.1] |
G6P ↔ F6P | 0.0 | [0.0, Inf] |
F6P → DHAP + GAP | 36.5 | [32.6, 40.7] |
DHAP → GAP | 35.5 | [31.6, 39.7] |
DHAP ↔ GAP | 0.0 | [0.0, 258.7] |
GAP → 3PG | 74.0 | [65.7, 82.8] |
GAP ↔ 3PG | 0.0 | [0.0, Inf] |
3PG → Pyr | 73.1 | [64.8, 81.9] |
Pyr → Lac | 73.6 | [65.8, 83.3] |
Pyr ↔ Lac | 13420 | [0.0, Inf] |
Lac → Lac.x | 73.6 | [65.8, 83.3] |
Pentose Phosphate Pathway | ||
---|---|---|
Reaction | Flux | Interval |
G6P → 6PG | 6.2 | [5.4, 7.4] |
6PG → P5P + CO2 | 6.2 | [5.4, 7.4] |
2 P5P → S7P + GAP | 2.0 | [1.8, 2.4] |
2 P5P ↔ S7P + GAP | 19290 | [0.0, Inf] |
S7P + GAP → F6P + E4P | 2.0 | [1.8, 2.4] |
S7P + GAP ↔ F6P + E4P | 0.0 | [0.0, 0.4] |
P5P + E4P → F6P + GAP | 2.0 | [1.8, 2.4] |
P5P + E4P ↔ F6P + GAP | 0.0 | [0.0, 0.6] |
TCA Cycle/Anaplerosis | ||
---|---|---|
Reaction | Flux | Interval |
Pyr + CO2 → OAA | 0.0 | [0.0, 1.7] |
Mal → Pyr + CO2 | 9.6 | [8.5, 10.7] |
Mal ↔ Pyr + CO2 | 1.3 | [0.9, 1.6] |
Pyr → AcCoAmit + CO2 | 8.6 | [5.5, 11.0] |
AcCoAmit + OAA → Cit | 8.6 | [5.5, 11.0] |
AKG + CO2 → Cit | 2.0 | [0.2,3.6] |
AKG + CO2 ↔ Cit | 5.7 | [4.6, 7.1] |
AKG → Suc + CO2 | 8.2 | [6.6, 10.7] |
Suc → Fum | 8.2 | [6.6, 10.7] |
Suc ↔ Fum | 0.2 | [0.0, Inf] |
Fum → Mal | 8.2 | [6.6, 10.7] |
Fum ↔ Mal | 23.8 | [5.2, Inf] |
OAA → Mal | 1.4 | [0.8, 2.9] |
OAA ↔ Mal | 106200 | [35.9, Inf] |
Amino Acid Metabolism | ||
---|---|---|
Reaction | Flux | Interval |
Glnext → Gln | 11.5 | [10.4, 12.6] |
Gln → Glu | 11.0 | [9.9, 12.1] |
Glu → AKG | 8.2 | [7.0, 9.3] |
Glu ↔ AKG | 81.3 | [35.9, 480.3] |
Pyr + Glu → Ala + AKG | 0.5 | [0.4, 0.6] |
OAA + Glu → Asp + AKG | 0.6 | [0.5, 0.7] |
3PG + Glu → Ser + AKG | 0.9 | [0.8, 1.1] |
Ser → Gly + MEETHF | 0.4 | [0.3, 0.4] |
Biomass Formation | ||
---|---|---|
Reaction | Flux | Interval |
P5P → NTP | 0.1 | [0.1, 0.1] |
DHAP → GLP | 1.0 | [0.8, 1.2] |
Cit → AcCoAcyt + OAA | 10.7 | [5.3, 14.5] |
AcCoAcyt → FA | 10.7 | [5.3, 14.5] |
Amino acids → Biomass | 3.3 | [2.7, 4.0] |