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. Author manuscript; available in PMC: 2011 Jan 25.
Published in final edited form as: J Biotechnol. 2009 Jul 19;144(3):167–174. doi: 10.1016/j.jbiotec.2009.07.010

Table 2.

Experimentally determined net (→) and exchange (↔) fluxes and 95% flux confidence intervals for the A549 carcinoma cell

Glycolysis

Reaction Flux Interval

Glucext → G6P 38.6 [34.7, 43.2]
G6P → F6P 32.4 [29.7, 35.1]
G6P ↔ F6P 0.0 [0.0, Inf]
F6P → DHAP + GAP 36.5 [32.6, 40.7]
DHAP → GAP 35.5 [31.6, 39.7]
DHAP ↔ GAP 0.0 [0.0, 258.7]
GAP → 3PG 74.0 [65.7, 82.8]
GAP ↔ 3PG 0.0 [0.0, Inf]
3PG → Pyr 73.1 [64.8, 81.9]
Pyr → Lac 73.6 [65.8, 83.3]
Pyr ↔ Lac 13420 [0.0, Inf]
Lac → Lac.x 73.6 [65.8, 83.3]
Pentose Phosphate Pathway

Reaction Flux Interval

G6P → 6PG 6.2 [5.4, 7.4]
6PG → P5P + CO2 6.2 [5.4, 7.4]
2 P5P → S7P + GAP 2.0 [1.8, 2.4]
2 P5P ↔ S7P + GAP 19290 [0.0, Inf]
S7P + GAP → F6P + E4P 2.0 [1.8, 2.4]
S7P + GAP ↔ F6P + E4P 0.0 [0.0, 0.4]
P5P + E4P → F6P + GAP 2.0 [1.8, 2.4]
P5P + E4P ↔ F6P + GAP 0.0 [0.0, 0.6]
TCA Cycle/Anaplerosis

Reaction Flux Interval

Pyr + CO2 → OAA 0.0 [0.0, 1.7]
Mal → Pyr + CO2 9.6 [8.5, 10.7]
Mal ↔ Pyr + CO2 1.3 [0.9, 1.6]
Pyr → AcCoAmit + CO2 8.6 [5.5, 11.0]
AcCoAmit + OAA → Cit 8.6 [5.5, 11.0]
AKG + CO2 → Cit 2.0 [0.2,3.6]
AKG + CO2 ↔ Cit 5.7 [4.6, 7.1]
AKG → Suc + CO2 8.2 [6.6, 10.7]
Suc → Fum 8.2 [6.6, 10.7]
Suc ↔ Fum 0.2 [0.0, Inf]
Fum → Mal 8.2 [6.6, 10.7]
Fum ↔ Mal 23.8 [5.2, Inf]
OAA → Mal 1.4 [0.8, 2.9]
OAA ↔ Mal 106200 [35.9, Inf]
Amino Acid Metabolism

Reaction Flux Interval

Glnext → Gln 11.5 [10.4, 12.6]
Gln → Glu 11.0 [9.9, 12.1]
Glu → AKG 8.2 [7.0, 9.3]
Glu ↔ AKG 81.3 [35.9, 480.3]
Pyr + Glu → Ala + AKG 0.5 [0.4, 0.6]
OAA + Glu → Asp + AKG 0.6 [0.5, 0.7]
3PG + Glu → Ser + AKG 0.9 [0.8, 1.1]
Ser → Gly + MEETHF 0.4 [0.3, 0.4]
Biomass Formation

Reaction Flux Interval

P5P → NTP 0.1 [0.1, 0.1]
DHAP → GLP 1.0 [0.8, 1.2]
Cit → AcCoAcyt + OAA 10.7 [5.3, 14.5]
AcCoAcyt → FA 10.7 [5.3, 14.5]
Amino acids → Biomass 3.3 [2.7, 4.0]
HHS Vulnerability Disclosure