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. Author manuscript; available in PMC: 2011 Jan 25.
Published in final edited form as: Mol Cell Pharmacol. 2010;2(4):131–136. doi: 10.4255/mcpharmacol.10.18

Figure 2. Oregon Green dextran (70 kD) localizes extensively in livers of CQ-treated and untreated mice.

Figure 2

Mice were dosed with 50 mg/kg/day chloroquine i.p. (or with normal saline vehicle control) for 5 days prior to a tail vein injection of 0.5 mg Oregon Green-labeled dextran. To visualize dextran localization, livers were extracted and imaged using the Maestro In Vivo Imaging system. Lysosomal pH of liver cells was found to be significantly elevated in mice treated with CQ. Lysosomal pH values were obtained by calibrating intracellular pH to known values using the ionophores nigericin (10µM) and monensin (20µM). The microscopically determined lysosomal pH values obtained from mice livers, with or without CQ treatment, are shown (pH values are mean ± SD, n=3).