Table 4.
cDNA Sequence Length | Open Reading Frame (ORF) | Stop Codon Toleration | |
---|---|---|---|
EXON_KB | Whole Length | Annotated ORF Only | Not Tolerated |
E_E_KB | ≤ 240 bp | Annotated ORF Only | Not Tolerated |
E_I_TH | ≤ 240 bp | All 3 ORFs | In 3’ end segment |
I_E_TH | ≤ 240 bp | All 3 ORFs | Not Tolerated |
E_E_TH | ≤ 240 bp | All 3 ORFs | In 3’ end segment |
We limited the lengths of the combination transcripts to 240 bp, with a flanking sequence of 120 bp on each side of the splice site.