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Data Generation Protocols
| cDNA Sequence Length | Open Reading Frame (ORF) | Stop Codon Toleration | |
|---|---|---|---|
| EXON_KB | Whole Length | Annotated ORF Only | Not Tolerated |
| E_E_KB | ≤ 240 bp | Annotated ORF Only | Not Tolerated |
| E_I_TH | ≤ 240 bp | All 3 ORFs | In 3’ end segment |
| I_E_TH | ≤ 240 bp | All 3 ORFs | Not Tolerated |
| E_E_TH | ≤ 240 bp | All 3 ORFs | In 3’ end segment |
We limited the lengths of the combination transcripts to 240 bp, with a flanking sequence of 120 bp on each side of the splice site.
