Table 2.
Formation and deamination of CmC dimers. 32P-labeled C in boldface. Mismatches are underlined.
| Entry | Abbrev. | sequence | % Yield | t1/2 |
|---|---|---|---|---|
| 1 | ACmCA | ACmCAt | 3.4 | nd |
| TG GTa | ||||
| 2 | GCmCG | GCmCGt | 1.4 | nd |
| CG GCa | ||||
| 3 | ACmCG | ACmCGt | 2.8 | 320 ± 12 |
| TG GCa | ||||
| 4 | ACmCG | ACmCGt | 3.0 | 256 ± 16 |
| TG GCa | ||||
| 5 | AUmCG | AUmCGt | 0.8 | 4.7 ± 1 |
| TG GCa | ||||
| 6 | ACTG | ACTGg | 3.9 | 28 ± 2.6 |
| TGGCc | ||||
| 7 | ACTG | ACTGg | 0.8 | 131 ± 12 |
| TGACc |
a
The NPyPyNn sequence shown is embedded in the 42-mer single strand sequence: d(GTGTATGTATGTGTATGTNXYNnCGTGTATATATGTGTATGT). The double strand sequence is formed by annealing to its fully complementary strand except were underlined.