Fig. 7.
ubp1-HGR converts foxi1-HGR-induced alpha-INCs into beta-INCs. (A-F) Xenopus embryos were injected at the 2-cell stage with foxi1-HGR or with both foxi1-HGR and ubp1-HGR RNA, along with mGFP RNA as a tracer. Injected embryos were treated with DEX at stage 12, fixed at stage 26, and then stained with B1 (red, upper panels) and with ae1 (blue, lower panels) antibodies. (G) pendrin-GFPtr embryos were injected with foxi1-HGR, ubp1-HGR and mGFP as tracer, and then stained with ae1 antibody (blue). (H) Based on ae1 and B1 staining, alpha-INCs and beta-INCs per field were scored under the various conditions; each field represents 196 μm2 of embryo surface. Error bars indicate ± s.d. Scale bar: 20 μm.