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Flagellin homologue as a cell surface alginate receptor. (A) Localization of flagellin homologue p5 in strain A1 cells by immunogold electron microscopy (upper, strain A1 cell grown on alginate; lower, strain A1 cell grown in the absence of alginate). (B) Interaction of p5 and alginate using an SPR biosensor. p5 purified from E. coli cells was immobilized on a sensor chip. The binding of p5 to alginate is dependent on alginate concentration (0.31–100 µg/ml).
