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Chick minimal infective dosage test result
| Groups | Tested chicks | Infective dosages | Infection route | Affected chicks |
| A | 5 | 0.2ml (5.60 × 1010ELD50) | Nasal drip | 3 |
| B | 5 | 0.3ml (8.4 × 1010ELD50) | Nasal drip | 5 |
| C | 5 | 0.4ml (1.1 × 1011ELD50) | Nasal drip | 5 |
| control | 5 | 0.4ml normal saline | Nasal drip | 0 |
