Table 6.
Functional responses of natural killer (NK) T cell lines
| Experiment 1 | Patient B2 | Patient B7 |
|---|---|---|
| Autologous tumour + αGalCer† | 0‡ | 0 |
| Autologous tumour | 1 | 1 |
| Autologous tumour lysate + C1R-huCD1d§ + αGalCer | n.t. | 3 |
| Autologous tumour lysate + C1R-huCD1d | n.t. | 2 |
| Controls | ||
| C1R-huCD1d + αGalCer | 26 | 15 |
| C1R-huCD1d | 2 | 7 |
| C1R + αGalCer | 1 | 0 |
| C1R | 1 | 0 |
| PMA/ionomycin | 11 (61¶) | 14 (54) |
| Medium | 0 | 0 |
| Experiment 2 | Patient B2 | Patient B7 |
|---|---|---|
| Autologous tumour + αGalCer | 0 | 7 |
| Autologous tumour | 0 | 0 |
| Autologous tumour lysate + C1R-huCD1d + αGalCer | 5 | 1 |
| Autologous tumour lysate + C1R-huCD1d | 1 | 0 |
| allogeneic tumour lysate†† + C1R-huCD1d + αGalCer | 5 | 1 |
| allogeneic tumour lysate + C1R-huCD1d | 1 | 0 |
| Controls | ||
| C1R-huCD1d + αGalCer | 7 | 8 |
| C1R-huCD1d | 4 | 0 |
| C1R + αGalCer | 0 | 0 |
| C1R | 0 | 0 |
| PMA/ionomycin | 22 (36) | 22 (23) |
| Medium | 0 | 0 |
†
α-Galactosylceramide (αGalCer).
‡
Interferon (IFN)-γ spots/well of 5 × 102 effector cells co-cultured overnight with 2 × 103 targets in enzyme-linked immunospot assay.
§
C1R cell line untransfected (C1R) or transfected with human CD1d (C1R-huCD1d).
¶
Within brackets, responses of allogeneic peripheral blood mononuclear cell effectors.
††
Lysates of the other NK T patient were used.
PMA: phorbol myristate acetate; n.t.: not tested.