Fig. 3.

The effect of cross-linking (X–L) granulocyte CD28 on surface expression of CD66 and CD28: kinetic studies. Purified granulocytes were incubated on a multimeric array of anti-CD28 (clone 204.12) or mouse IgG1 antibody as a control, at 37°C for various time-intervals prior to measurement of cell surface CD66 (clone Kat4c) and CD28 (clone 15E8) by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated antibodies. Results are shown as the mean fluorescence intensity (MFI). Mean ± standard error of the mean of three experiments.