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. 2010 Sep 15;30(37):12466–12473. doi: 10.1523/JNEUROSCI.3141-10.2010

Figure 1.

Figure 1.

Targeting Phox2b-expressing ventrolateral brainstem neurons using PRSx8-AlstR-EGFP-LV. A, Confocal images of Phox2b-immunoreactive nuclei (red) expressing AlstR-EGFP (green). Colocalization appears as yellow. Bregma level, −11.3 mm. B, Distribution of Phox2b neurons expressing AlstR-EGFP transgene in one representative rat 5 weeks after microinjection of PRSx8-AlstR-EGFP-LV into the ventrolateral brainstem. Each symbol represents three neurons. Numbers on the right indicate distances from bregma. C, Phox2b-immunoreactive neuron expressing AlstR-EGFP residing within the VMS marginal layer. 7, Facial motor nucleus; py, pyramidal tract. Scale bars, 50 μm.