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. Author manuscript; available in PMC: 2011 May 12.
Published in final edited form as: Cell. 2010 Nov 12;143(4):579–591. doi: 10.1016/j.cell.2010.10.028

Figure 1. Bypassing ERAD components by Hrd1p overexpression.

Figure 1

(A) The degradation of the misfolded luminal ER protein CPY*-HA was followed after inhibition of protein synthesis by cycloheximide in wild type (wt) cells or in cells overexpressing Hrd1p under the GAL promoter in the presence of galactose. Where indicated, genes for ERAD components were deleted. The graph shows quantification of the data.

(B) As in (A), but with overexpression of Der1p. The overexpression was confirmed by immunoblotting with specific antibodies (not shown).

(C) As in (B), but with overexpression of Usa1p.

(D) As in (B), but with overexpression of Hrd3p.

See also Figure S1 and Figure S2.