Skip to main content
. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: J Am Coll Cardiol. 2010 Dec 14;56(25):2115–2125. doi: 10.1016/j.jacc.2010.07.033

Fig.4. CBD inhibits diabetes-induced myocardial, iNOS expression and 3-NT accumulation.

Fig.4

Fig.4

(A) iNOS expression was determined by Western immunoblot in the heart tissues (B) 3-NT levels in the heart samples were quantitatively determined by ELISA with indicated CBD concentration (mg/kg body weight) respectively. (C) Representative gel indicating the nitrated proteins analyzed by immunoprecipitation with 3-NT specific antibody (D) shows the representative images for the histochemical staining for 3-NT levels in the formalin-fixed myocardial tissues. (E) Depicts the immunofluorescence staining for 3-NT from frozen sections as described in methods. *P<0.05 vs. vehicle control/CBD alone; #P<0.05 vs. diabetes [D], n= 6-8/group.