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Primers used to amplify rye homologues of embryogenesis-related genes
| Gene | Primer | Sequence 5’-3’ | Size of product [bp] |
|---|---|---|---|
| SERK | SERK_z | F: TTGCTGGAGGTGTTGCTG R: TACACCTTTCCAAAGCCAC | 234 |
| LEC1 | AU088 | F: CAAGGAGACGATCCAGGAGT R: GGTAGCGGTGGAGGTAGACG | 180 |
| VP1 | VP | F: AGAAGGTGCTGAAGCAGAGC R: CCCTGTGTTTTCCAGCAGAT | 203 |
| NiR | NiR_zyt | F: GAGAAGAGGATGCCGAACG R: ATGTTCTGCTCCACGGTGA | 235 |
| Control 18 S rRNA | 18 S | F: CATCCCAAAGTCCAACTA R: GCTACCACATCCAAGGAA | 250 |
