Fig. 1.

Effect of δ-aminolevulinic acid (δ-ALA), betaine, glycerol, and taurine on oligomerization of 27 cystathionine β-synthase (CBS) mutants. Crude extracts—15 ·g total protein—obtained from the cells grown in the absence (odd lanes) and presence (even lanes) of chaperone were examined for the presence of CBS tetramers/oligomers by native polyacrylamide gel electrophoresis (PAGE) and Western blot with immunodetection. Correctly assembled CBS tetramers (arrows) and higher-order oligomers (octamers, dodecamers, hexadecamers) are present as sharply demarcated fractions. Mutants responding to chaperones by formation of increased amounts of correctly assembled CBS molecules are shown in boxes