Figure 10. Time to thrombus resolution in calcium ionophore–treated mesenteric venules.

Upon calcium ionophore stimulation of the mesenteric venules, platelets immediately began to accumulate on the vessel wall. Adhesion was monitored, and the time required for the fluorescence value to return to baseline was measured. Platelet adhesion persisted longer in mice deficient in ADAMTS13 than in wild-type mice. NAC treatment in either ADAMTS13^–/– or wild-type mice significantly shortened the time required for platelet adhesion to return to baseline. The mean (± SEM) values for time to thrombus resolution were as follows. ADAMTS13^–/–: control, 10.4 ± 1.2 minutes; NAC, 5.1 ± 0.3 minutes; wild type: control, 6.5 ± 0.5 minutes; NAC, 3.8 ± 0.3 minutes. 10 mice were examined in each group. The data were analyzed using Student’s t test, and the adjusted P values are indicated.