Figure 9. Platelet thrombus accumulation in ADAMTS13^–/– mice with and without NAC treatment.

ADAMTS13^–/– mice were injected with fluorescently labeled platelets from mice of the same genotype, and their mesenteric vessels were then exposed and treated with calcium ionophore to induce vWF secretion. Platelet accumulation in the vessels was monitored microscopically. Upper panels: sequential images taken at the indicated times after the application of calcium ionophore to ADAMTS13^–/– mice with or without NAC treatment. Images taken at similar times from the 2 mice are aligned. Original magnification, ×200. Lower panel: MFI of platelet thrombi was quantified on each frame of the recorded movie and plotted against time.