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. 2011 Jan 18;121(2):683–694. doi: 10.1172/JCI42314

Figure 5. Tumor protection induced by α-GalCer and C20:2, but not β-ManCer, is dependent on IFN-γ.

Figure 5

CT26 cells (5 × 105 cells) were injected i.v. into the tail vein of BALB/c WT or Ifng–/– mice on day 0, and glycolipids (50 pmoles) were administered within 1 hour after tumor challenge. Mice (5 mice per group) were sacrificed 14–16 days after tumor challenge, and lung metastases were enumerated. (A) WT (black symbols) or Ifng–/– (white symbols) were used. *P < 0.05 compared with respective vehicle control. (B) WT BALB/c mice were treated with 200 μg anti-CD8 antibodies (white symbols) or rat IgG control antibodies (black symbols) on days –1, 0, 5, and 10. *P < 0.05 compared with vehicle control. (C) BALB/c mice were treated 25 μl anti-asialo GM1 antibodies (white symbols) or control rabbit serum (black symbols) on days –1, 0, 5, and 10. *P < 0.05 compared with respective groups. Representative experiments of at least 2 repeats are shown. Vertical bars indicate the interquartile range, and horizontal bars indicate the median value. Each symbol represents an individual mouse.