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(A) Chiral separation of 18S- and 18R-RvE2 after 5-LOX incubation and reduction. Diastereomeric mixture of RvE2 was isolated by conventional RP-HPLC separation and further resolved by chiral HPLC. (B) Tandem mass spectra of 18R-RvE2 and 18S-RvE2 and (C) their MS/MS assignment. 18R- and 18S-RvE2 had indistinguishable MS/MS spectra.
