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. 2011 Jan 4;121(2):569–581. doi: 10.1172/JCI42545

Figure 5. LTA4H in RvE1 biosynthesis.

Figure 5

(A) Activated human PMNs were incubated with 18-HEPE, in the presence or absence of LTA4H inhibitor bestatin, and representative LC-MS/MS chromatograms, transition of 349→205 monitored, are shown. Treating PMNs with hydrolase inhibitor (250 μM bestatin) reduced RvE1 synthesis by 67.3% ± 6.2% (mean ± SEM, n = 3; P < 0.05 compared with control). (B) LC-MS/MS spectrum of RvE1 from 5-LOX (25 U) and LTA4H (2 μg) combinatorial incubation. Retention time of this product matched with synthetic RvE1. Statistically significant amounts of RvE1 were not produced with 5-LOX alone or with bestatin-treated combinatorial incubations.