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. 2011 Jan 4;121(2):739–751. doi: 10.1172/JCI42656

Figure 8. CD4+ T cell–independent CTL and antibody responses induced by Ad-siA20-BM-DCs.

Figure 8

Groups of C57BL/6 mice (n = 6) were treated with anti-CD4 antibodies (GK1.5, 200 μg/mouse, i.p. injection) 3 days before each immunization (44) and then immunized twice with gp120-pulsed (100 μg/ml), Ad-transfected DCs matured with LPS at a weekly interval, followed by in vivo stimulation with poly(I:C) (i.p., 50 μg/mouse) daily for 3 consecutive days. Splenocytes pooled from immunized mice were subjected to ICS (A), IFN-γ ELISPOT (B), and cytolytic assays against gp120-pulsed syngeneic TC1 cells (36) (C). HIV gp120–specific IgG antibody titers from pooled sera were analyzed by ELISA (D). Data from 1 of 2 repeated experiments are presented. *P < 0.05, Ad-siGFP-BM-DCs versus Ad-siA20-BM-DCs.