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. 2011 Jan 25;121(2):658–670. doi: 10.1172/JCI42974

Figure 5. LXR agonists suppress expression of RORγt and other Th17-related genes.

Figure 5

(A) Naive CD4+ T cells were cultured under Th17-inducing conditions for 4 days in the presence of vehicle control (C), T0901317 (T), and GW3965 (G) before cells were harvested for real-time PCR analysis of the genes as indicated above. Data are expressed as mean ± SD, and this experiment was repeated at least 3 times with similar results. *P < 0.05; **P < 0.01. (B) Naive CD4+ T cells isolated from RORγt WT mice or RORγt KO mice were cultured under Th17-inducing conditions with or without LXR agonists T0901317 (0 μM, 0.5 μM, 1 μM, 2 μM) and GW3965 (0 μM, 2.5 μM, 5 μM, 10 μM) for 4 days and subjected to intracellular staining of IL-17 and IFN-γ. This experiment was repeated at least 3 times with similar results. Values in the quadrants indicate the percentage of IL-17+IFN-γ– cells, IL-17+IFN-γ+ cells, and IL-17–IFN-γ+ cells.