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. 2011 Jan 4;121(2):671–682. doi: 10.1172/JCI43302

Figure 1. 5-LO metabolites are required for MyD88-dependent inflammatory responses in vitro and in vivo.

Figure 1

(A) Overview of LT biosynthesis and receptors. (B–D) RANTES (B), IL-6 (C), and nitrite (D) levels from WT- and 5-LO–/– elicited peritoneal macrophages stimulated for 24 hours as indicated, determined by ELISA or the Griess reaction. Cytokine and nitrite data represent mean ± SEM from 3 individual experiments, with each value determined in triplicate. *P < 0.05 versus control; #P < 0.001 versus WT. (E) WT and 5-LO–/– mice (5 mice per group in each of 2 independent experiments) were challenged with LPS or TNF-α i.p. as described in Methods, and survival was assessed over time. *P < 0.05 versus LPS-treated WT. (F–H) Levels of RANTES (F), IL-6 (G), and IL-10 (H) from WT and 5-LO–/– mice challenged with LPS or TNF-α, determined by ELISA on serum collected 6 hours after challenge. Cytokine data represent mean ± SEM from 3–5 animals per group in each of 3 individual experiments, with each value determined in triplicate. *P < 0.05 versus WT; #P < 0.05 versus WT.