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. 2011 Jan 18;121(2):715–725. doi: 10.1172/JCI43366

Figure 1. α-Syn is secreted into the medium of cultured cells.

Figure 1

(A, left) HEK cells not expressing (–) or expressing α-syn fused to GFP or DsRed and 30 μl of conditioned medium from these cells (c.m.). Expression was compared with 2 μg of recombinant α-syn. (A, right) Different concentrations of recombinant α-syn were compared with conditioned medium from 5 different dishes of GFP–α-syn–expressing HEK cells (c.m. 1 to 5). The average concentration of α-syn in the conditioned medium was estimated at 29 ± 3 nM (± SEM). (B) SH-SY5Y cells expressing α-syn fused to GFP or DsRed and 30 μl of conditioned medium from these cells. (C) Comparative Western blots showing expression levels of GFP–α-syn in HEK and SH-SY5Y stable transfectants (left) or in conditioned medium taken from these cells (right). The molecular weights are expressed in kDa on the left side of the blots.