Figure 3. Chimeras are tolerant to donor alloantigen.

Lymphocytes harvested from spleens and lymph nodes of naive mice, injected non-chimeras, and chimeras were labeled with CFSE and injected into (A–C) B6 × BALB/c or (D) C3H × DBA/2 (third-party control) F₁ recipients. Representative flow cytometric histograms showing CFSE profiles after gating on donor-derived (A) CD4⁺ or (B) CD8⁺ T lymphocytes are shown. (C) Percentage of alloreactive T cells in naive mice, non-chimeras, and chimeras. The data shown are representative of at least 4 independent experiments (naive, n = 10; non-chimera, n = 7; chimera, n = 10). *P < 0.05 comparing chimeras with naive mice and non-chimeras using ANOVA with Tukey’s multiple comparison test. (D) Percentage of donor-derived proliferating cells (%CFSE^low) from naive and chimeric mice in response to a third-party antigen. Data are representative of at least 2 independent experiments (naive, n = 3; chimera, n = 5).