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. 2011 Jan 25;6(1):e14576. doi: 10.1371/journal.pone.0014576

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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a: mRNA data expressed as number of copies of adult or tadpole globin mRNA detected by real time PCR across various tadpole and metamorphic stages. Mean copy numbers were normalised using a toad actin housekeeping gene. Animals were staged according to Limbaugh and Volpe, 1957. Toadlet (*) development was approximately one month post-metamorphosis. b: Detection of globin proteins as determined by western blot analysis using specific antibodies to tadpole and adult globins. Coomassie staining indicates the loading level for each lane. Recombinant proteins for adult and tadpole globin (rAdglob and rTadglob, respectively), as well as native adult globin (Adult) were included as positive controls.