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. 2011 Jan 25;6(1):e14576. doi: 10.1371/journal.pone.0014576

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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200 ng protein per well from lysed red blood cell samples taken from metamorphs then stained with silver stain. a: Lanes 1 and 12, See Blue Plus2 Pre Stained Standard (Invitrogen); Lanes 2–4, blood from 3 control animals bathed in 10² TCID₅₀/ml of rBIV/neo^r; Lanes 5–10, blood from 6 test animals bathed in 10² TCID₅₀/ml of rBIV/neo^r/adglo. Lane 11, positive control, globin from a 2 month old toadlet. b: Panel shows silver stain and Western blot antibody detection of adult globin; lane i See Blue Plus2 Pre Stained Standard (Invitrogen); lane ii, positive control, globin from a 2 month old toadlet; lanes iii, iv and v, 500, 1000 and 5000 ng of protein from fluid of untreated stage 42 tadpoles.