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. 2011 Jan 25;6(1):e16026. doi: 10.1371/journal.pone.0016026

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Young et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot analysis of c-Fos protein in Pyk2^+/+ and Pyk2^−/− osteoblasts exposed to 30 minutes of FF (F) or maintained in static culture conditions (S). (B) Western blot analysis of c-Fos protein in FAK^+/+ and FAK^−/− osteoblasts exposed to 30 minutes of FF (F) or maintained in static culture conditions (S). Vinculin was analyzed as a loading control. Graph represents quantification of c-Fos protein levels expressed as raw densitometry units. Experiments were performed in triplicate, and n≥3 for each experimental group in each trial. Error bars represent standard error. ^aStatistically significant difference between static and FF (p<0.05).