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. 2011 Jan 25;6(1):e16026. doi: 10.1371/journal.pone.0016026

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Young et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression of OPN mRNA as determined by qRT-PCR analysis of Pyk2^+/+ and Pyk2^−/− osteoblasts exposed to either 2 hour of FF followed by a 20 hour post-flow incubation or maintained in static culture conditions. (B) Expression of OPN mRNA as determined by qRT-PCR analysis of FAK^+/+ and FAK^−/− osteoblasts exposed to either 2 hour of FF followed by a 20 hour post-flow incubation or maintained in static culture conditions. (C) Graphical representation of data in Fold Difference. Experiments were performed in triplicate, and n≥3 for each experimental group in each trial. Error bars represent standard error. ^aStatistically significant difference between static and FF (p<0.05). ^bStatistically significant difference between static conditions (p<0.05).