Figure 5. Extracellular pH sensitivity of concatenated constructs of WT and pH_o-insensitive TASK-2 channels.

The graph in A shows extracellular pH-dependence curves of WT TASK-2 (circles) and its mutant TASK-2-R224A (triangles), taken from [20]. Also shown are data for WT-WT (n = 4) and R224A-R224A (n = 6). In B the data for WT TASK-2 monomer are repeated for comparison with the pH-dependence curves of WT-R224A (n = 6) and R224A-WT (n = 6) mixed concatenated constructs. Results are shown as means ± SEM. The lines are fits of the Hill equation constructed using the average of fitted parameters of the individual experiments.