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. 2011 Jan 25;6(1):e16256. doi: 10.1371/journal.pone.0016256

Figure 3. Analysis of amino acid and cyanogenic content in cassava tissues transgenic for zeolin.

Figure 3

A) Total amino acid profile of storage roots of non-transgenic cassava cv. 60444 (green bars) and transgenic line pILTAB600-25 (blue bars) in which zeolin is driven by the patatin promoter. Increase in amounts of individual amino acids in transgenic root tissue varied from a four- to ninefold increase compared to the non-transgenic control in a manner that reflects the expect amino acid profile for zeolin. B, C) Levels of cyanogens present in cassava leaf and root tissues of seven-month-old, pot grown plants. All transgenic lines showed a reduction in cyanogen content within leaves and storage roots compared to the non-transgenic control and was apparent in transgenic events in which zeolin was under control of either the patatin (pILTAB600, blue bars) or 35S (pILTAB601, orange bars) promoters. A maximum reduction of 55% was observed in roots of pILTAB600-25, the transgenic event that accumulated the highest levels of total protein within its storage roots.