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A. Validation of novel miRNAs by Stem-loop RT-PCR; B. The expression profile of ssc-miR-62 was detected by Northern blotting. Total RNA isolation from porcine heart, liver, spleen, lung, kidney, skeletal muscle, stomach, small intestine, lymph node and brain, 5S rRNA served as a loading control. Hybridization signals were detected for mature new miRNA.
