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. 2011 Jan 25;6(1):e16433. doi: 10.1371/journal.pone.0016433

Figure 2. Flagellin is delivered to apical epithelial cells across the stratum corneum and induces psoriasin expression.

Figure 2

A: Immunohistochemical analyses of psoriasin expression (Vector red) in full-thickness ex vivo skin explants. One representative experiment is shown (n = 3–6). Biopsies were incubated with P. aeruginosa wildtype (PAK) and its flagellin deletion mutant (ΔfliC) in transwells for 24 h. B: Flagellin Western Blot analysis of the correspondent culture supernatants. C: Rhamnolipid expression of P. aeruginosa grown directly on ex vivo skin was assessed by rhlA mRNA expression. A representative gel out of three independent experiments is shown with a 100 bp ladder (lane1), reverse transcription PCR products of PAK (lane 2), ΔfliC (lane 3), and untreated skin explants (lane 4), a PCR control for DNA contamination (lane 5) and the PCR product using PAK genomic DNA (lane 6).