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. 2011 Jan 25;6(1):e16433. doi: 10.1371/journal.pone.0016433

Figure 5. Effects of acetone and different detergents on flagellin-induced psoriasin expression in human skin.

Figure 5

. For immunohistochemical analyses of psoriasin expression (Vector red) in full-thickness ex vivo skin explants, biopsies were incubated with the different surfactants (the anionic detergent DOC, the zwitterionic CHAPS, the cationic CTAB, and the nonionic TWEEN 20) in DMEM for 48 h in presence and absence of P. aeruginosa flagellin. Surfactants were added above their CMC. The surfactant net charge is given in brackets. To disorder the physical barrier, ex vivo skin was treated with acetone prior incubation with flagellin. One representative experiment out of three is displayed.