Fig. 5.

ITCs induce cell cycle arrest and apoptosis in MM cells. (A) U266 cells were treated with 10 μM ITCs or 40 nM Bortezomib for 24 h followed by cell cycle analysis. White bars: G₁ phase; striped bars: S phase; black bars: G₂/M phase. (B) ITCs induce substantial amount of sub-G₁ (apoptotic) cells. (C) BITC induced poly (ADP-ribose) polymerase cleavage. U266 cells were treated with up to 15 μM BITC for 24 h before detection of both full length and cleaved poly (ADP-ribose) polymerase by immunoblots. *P < 0.05; **P < 0.01; ***P < 0.001.