Skip to main content
. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: Immunol Lett. 2010 Nov 19;135(1-2):173–179. doi: 10.1016/j.imlet.2010.11.004

Figure 2.

Figure 2

Representative EMSA demonstrating the differential effects of N-terminus Hsp72 and C-terminus Hsp72 on NF-κB-DNA binding. RAW 264.7 macrophages weretreated with the indicated conditions for 1 hour and then cells were harvested for isolation of nuclear proteins. Control cells were treated with media. As a positive control one group of cells was treated with LPS (100 ng/ml). To control for LPS contamination of the Hsp72 preparations a separate group of cells was treated with an equivalent amount of LPS (0.02 ng/ml) as that found in the highest concentration of Hsp72. Cold competitor and supershift assays were performed as described in the Methods section. The gel is representative of 3 different experiments with similar results.