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. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: Immunol Lett. 2010 Nov 19;135(1-2):173–179. doi: 10.1016/j.imlet.2010.11.004

Figure 8.

Figure 8

Luciferase assay demonstrating the effect of dosing with equimolar concentrations of N-terminus and C-terminus Hsp72. RAW 264.7 macrophages were transiently transfected with a NF-κB-dependent luciferase reporter plasmid and treated with the indicated conditions for 4 hours. As a control for transfection efficiency, cells were co-transfected with a Renilla luciferase control reporter plasmid. Control cells were treated with media. The other groups were treated with 4.2 pmol N-terminus or C-terminus Hsp72 as indicated. Data are expressed as mean fold induction over control (± S.E.M.) of luciferase activity over control cells and are normalized for the respective Renilla luciferase activity. Data represent 3 individual experiments, with each experimental condition performed in duplicate within each experiment. *p < 0.05 versus control (ANOVA with Bonferroni correction for multiple comparisons).