Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The embryonic stem cell test (EST) established for mESCs by Spielmann and co-workers. The EST comprises three parameters, the differentiation of mES cells (endpoint 1), cytotoxic effects on ES (D3) cells (endpoint 2) and on NIH 3T3 fibroblasts (endpoint 3). On the basis of these data, concentration response curves were established and the prediction for embryotoxic effects of compounds calculated (according to Seiler et al. 2006; see text for further information)
