Common Homologous Recombination (HR) |
Zwaka and Thomson (2003) |
hES (H1.1) |
HPRT1; OCT4 |
Proof-of-concept for homologous recombination in hES cells |
|
Urbach et al. (2004) |
hES (H13) |
HPRT1 |
Modelling of Lesch-Nyhan syndrome in hES cells |
|
Irion et al. (2007) |
hES (HES-2) |
Rosa 26 human equivalent |
Definition of a site suitable for gene transfer in hES cells |
|
Davis et al. (2008) |
hES (HES-3) |
MIXL1 |
Establishment of cell lines for lineage selection in hematopoietic differentiation; Cre-lox-mediated excision of marker genes |
|
Suzuki et al. (2008) |
hES (KhES1, KhES3) |
HPRT1 |
Proof-of-concept of adeno-associated virus (AAV)-based gene transfer for HR in hES cells |
|
Mitsui et al. (2009) |
hES (KhES1; KhES3) hiPS (243H7; 246H1) |
HPRT1; NANOG |
Proof of concept for efficiency of HR in hES cells using adeno-associated virus-based gene transfer vectors |
|
Ruby and Zheng (2009) |
hES (HUES9) |
FEZF2 |
Proof-of-concept for effective HR in HUES9 cells, establishment of cell lines for lineage selection of subcortical projection neurons |
|
Xue et al. (2009) |
hES (BG01) |
OLIG2 |
Establishment of cell lines for lineage selection in neural differentiation |
|
Khan et al. (2010) |
hES (H1; BG01; BG02) hiPS (several clones) |
HPRT1; HMGA1; Fibroblasts subsequently used for hiPS cell generation |
Proof-of-concept of AAV-based gene transfer for HR in hES and hiPS cells; hiPS cell generation from human cells subsequent to gene targeting |
|
Song et al. (2010) |
hES (HUES3, HUES8, HUES9) |
P53, ATM1; HPRT1 |
Establishment of p53−/− and ATM−/− hES cell lines; use of bacterial artificial chromosome (BAC)-based targeting vectors |
Zinc finger nuclease (ZFN)—enhanced homologous recombination |
Lombardo et al. (2007) |
hES (HUES1, HUES3) |
CCR5 |
Proof-of-concept of zinc-finger nuclease (ZFN)–enhanced HR in multiple human cell types including hES cells; use of an integrase-defective lentiviral vector for gene transfer |
|
Hockemeyer et al. (2009) |
hES (BG01); hiPS (several clones) |
Oct4; PP1R12C (AAVS1 locus); PITX3 |
Proof-of-concept for zinc-finger nuclease (ZFN)–enhanced HR into expressed and non-expressed sites in hES cells |
|
Zou et al. (2009) |
hES (H9, H1) hiPS (two lines) |
Lentivirally transduced mutated eGFP gene; PIG-A |
Proof-of-concept for zinc-finger nuclease (ZFN)–enhanced HR in hES cells; targeting of a disease-related gene in hES cells |
Site-specific (phage-derived) recombination systems |
Di Dominico et al. (2008) |
hES (H1) |
HPRT1 |
Establishment of a Cre/Lox P-based gene replacement system; proof-of-concept for effective recombinase-mediated cassette exchange |
|
Thyagarajan et al. (2008) |
hES (BG01v) |
Pseudo attP sites in the human genome |
Proof-of-concept for functionality of the Streptomyces phage phiC31-based recombination system in hES cells |
|
Sakurai et al. (2010) |
hES (KhES1) |
HPRT1 |
Establishment of a Cre/Lox P-based gene replacement system |