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. 2010 Dec 31;10(6):219–229. doi: 10.4110/in.2010.10.6.219

Figure 1.

Figure 1

PMA stimulation of U937 cells induces IL-10 production. (A) Dose- and time-dependent increase in IL-10 mRNA after PMA stimulation. U937 cells were stimulated with PMA for different time periods and harvested for mRNA analysis. Transcript levels of IL-10 and β-actin (loading control) were determined by RT-PCR. (B) U937 cells were stimulated with different concentrations of PMA for 24 h. Cells were harvested, and IL-10 protein levels in the supernatants were determined by ELISA. Data represent the mean±SD of duplicates.