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. 2010 Dec 31;10(6):219–229. doi: 10.4110/in.2010.10.6.219

Figure 4.

Figure 4

NDRG2 expression inhibits induction of SOCS3 but promotes STAT3 phosphorylation after PMA stimulation. (A) U937-mock and U937-NDRG2 cells were stimulated with 50 ng/ml of PMA for 15 or 30 min. Cell lysates were harvested and equal amounts of lysates were subjected to electrophoresis on SDS-PAGE. Western blotting was performed using STAT3-, pSTAT3- and NDRG2-specific antibodies. (B) Cells were stimulated at various times with 50 ng/ml of PMA. Equal amounts of whole-cell lysates were subjected to electrophoresis on SDS-PAGE, and then western blotting was performed using specific antibodies.