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. 2010 Dec 31;10(6):219–229. doi: 10.4110/in.2010.10.6.219

Figure 5.

Figure 5

Inhibition of STAT3 phosphorylation increases IL-10 production. (A) U937-mock cells were pretreated with cucurbitacin I for 2 h followed by PMA treatment for 48 h. Cells were harvested for mRNA analysis. Transcript levels of IL-10 were determined by RT-PCR. (B) U937-NDRG2 cells were pretreated with cucurbitacin I or AG490 for 2 h followed by PMA treatment for 48 h. Cells were harvested for mRNA analysis. Transcript levels of IL-10 were assessed by RT-PCR. (C) U937-NDRG2 cells were treated in the same manner as in (B). The levels of IL-10 in culture supernatants were determined by ELISA. Data represent the mean±SD of duplicates.