Figure 5.

Confirmation of RGS1 GeneChip expression data using RT-PCR. The same RNA obtained from MS patients treated with Betaseron and used for the GeneChip study was used to independently validate the expression of RGS1 using RT-PCR. RGS1 expression was determined using RT-PCR (TaqMan custom primer set Hs00175260_m1) at 0, 4, 18, and 42 hours post-Betaseron administration. MS 1–7 refers to MS patients included in the initial study. Data are expressed as linearized C(t) values normalized to GAPDH. Data are presented as the mean ± SD (n = 3).