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PCR sequencing and restriction digest for the canine mutation. (a) Sequencing chromatogram of the mutation region showing the G450C transversion on both alleles of an affected dog (−/−) and on one allele in a heterozygote (+/−). (b) Ncol digest after amplification of the target region resulting in cutting of the wild-type alleles (+/+), cutting of one allele in the heterozygote (+/−), and larger undigested bands from both alleles in the homozygous dog (−/−).
