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Los1p facilitates nuclear re-export of retrogradely transported spliced tRNAs. BY4742 pYX242, BY4742 pYX242-UTP8, BY4742 pYX242-CEX1 and BY4742 pYX242-LOS1 were starved of nitrogen in synthetic medium containing glucose and all amino acids except for leucine. At the specified times, FISH was used to monitor the cellular location of tRNATyr and tRNAGly using Cy3-labelled oligonucleotides described previously.25
