Figure 4.

Rapamycin treatment does not affect nuclear and cytoplasmic aminoacylation of tRNA^Tyr. The TB50a strain was incubated with drug delivery solution with or without 200 ng/ml rapamycin in YPD medium for 1 h and nuclear and cytoplasmic fractions were isolated.²⁵ Total RNA from each fraction was isolated and subjected to northern blot analysis to detect tRNA^Tyr.²⁵ Deacylated tRNAs were prepared by incubating total RNA from nuclear and post-nuclear fractions in 100 mM Tris-HCl, pH 9.5 at 37°C for 1 h.