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. 2010 May 21;1(4):354–366. doi: 10.4161/nucl.1.4.12435

Figure 3.

Figure 3

Design and results of the yeast rescue experiment. (A) Systematic view of the rescue experiment. (B) Analysis of human LBR constructs by GC/MS. hLBRwt is able to complement ERG24 deficiency in yeast. hLBR_N547D is partially able to complement ERG24 deficiency, visible in the production of the end product of the sterol pathway, ergosterol. However, providing this essential component happens on the cost of also producing large amounts of the abnormal metabolites 4-methylzymosterol and ignosterol. hLBR_R583Q is not able to complement ERG24 deficiency, seen in the insufficient production of the normal endproduct ergosterol and massive accumulation of the irregular product ignosterol. (C) Growth pattern. Mutation p.R583Q failed to normalize the growth of Erg24 deficient yeast. Mutation p.N547D partially compensated for Erg24 deficiency with respect to growth.