Figure 4.
Downregulation of polyprenyl synthetase by fdps-1(RNAi) blocks the redistribution of chromatin in nuclei of aging cells. Animals were treated with either L4440 vector (EV) or with fdps-1(RNAi) starting at the young adult stage (day 1). Ce-emerin::GFP and Hoechst DNA staining were used to view changes in nuclear envelope shapes and chromatin distribution. fdps-1(RNAi)-treated worms show diminished chromatin mislocalization at old age. In the merge parts green = lamin; blue = DNA. Bars = 10 microns.